Antigen

Antigen Class

TFs and others

Antigen

NUTM1

Cell type

Cell type Class

Pluripotent stem cell

Cell type

hESC derived mesendodermal cells

NA

NA

Sample

source_name

Cell state-dependent chromatin targeting in NUT carcinoma

cell line

HUES64 derived dME

cell type

differentiated cells

chip antibody

NUT (C52B1) Rabbit mAb#3625

geo_loc_name

missing

collection_date

missing

Sequenced DNA Library

library_name

GSM7165514

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Formaldehyde cross-linked chromatin for ChIP-seq experiments with antibodies against were prepared from 5 × 107 transient transfected cells of each HUES64, HUEs64 derived mesoderm, Ntera-2 and Ntera-2 derived differentiated cells as described in Alekseyenko et al. 2015. ChIP-seq was performed using anti-NUT (15 ul per IP; Cell Signaling, catalog no. 3625).ChIP-seq library preparation were performed as described (Alekseyenko et al. 2015). ChIP-seq library preparation were performed as described (Alekseyenko et al. 2015).

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

19075058

Reads aligned (%)

97.6

Duplicates removed (%)

3.1

Number of peaks

812 (qval < 1E-05)

hg19

Number of total reads

19075058

Reads aligned (%)

97.0

Duplicates removed (%)

4.2

Number of peaks

859 (qval < 1E-05)