Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
NUTM1

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC derived mesendodermal cells
NA
NA

Attributes by original data submitter

Sample

source_name
Cell state-dependent chromatin targeting in NUT carcinoma
cell line
HUES64 derived dME
cell type
differentiated cells
chip antibody
NUT (C52B1) Rabbit mAb#3625
geo_loc_name
missing
collection_date
missing

Sequenced DNA Library

library_name
GSM7165514
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Formaldehyde cross-linked chromatin for ChIP-seq experiments with antibodies against were prepared from 5 × 107 transient transfected cells of each HUES64, HUEs64 derived mesoderm, Ntera-2 and Ntera-2 derived differentiated cells as described in Alekseyenko et al. 2015. ChIP-seq was performed using anti-NUT (15 ul per IP; Cell Signaling, catalog no. 3625).ChIP-seq library preparation were performed as described (Alekseyenko et al. 2015). ChIP-seq library preparation were performed as described (Alekseyenko et al. 2015).

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
19075058
Reads aligned (%)
97.6
Duplicates removed (%)
3.1
Number of peaks
812 (qval < 1E-05)

hg19

Number of total reads
19075058
Reads aligned (%)
97.0
Duplicates removed (%)
4.2
Number of peaks
859 (qval < 1E-05)

Base call quality data from DBCLS SRA